Effect of the pro-gly dipeptide on the zoosocial behavior of outbred and inbred mice

The aim of the work was to study the effect of Pro-Gly (a dipeptide structurally similar to the Pro-Leu oxytocin fragment) on the zoosocial behavior of laboratory rodents.The studies were performed on adult outbred ICR male mice, inbred C57Bl/6 and BALB/c male mice. The effect of Pro-Gly on muscle strength and the level of situative anxiety in ICR mice was determined. The effect of Pro-Gly on zoosocial behavior of ICR mice was studied in a three-chamber apparatus for assessing the socialization. The effects of the studied dipeptide were evaluated in the conditions of model pathology caused by the administration of a non-competitive blocker of N-methyl-D-aspartate glutamate receptors - MK-801 to C57Bl/6 and BALB/c mice.Pro-Gly significantly increased sociability in rodents without behavioral disorders (outbred ICR and inbred C57Bl/6 mice). ICR mice after oral administration of Pro-Gly (0,5 mg / kg) spent 40,4% more time in the chamber with mouse than mice of the control group (398,5±42,7 s versus 283,9±42,9 s, respectively), and they preferred a camera with a mouse (398,5±42,7 s), rather than a camera with a Lego (89,9±38,3 s; p<0,05). The dipeptide significantly increased the sociability of C57Bl/6 mice: individuals of this strain preferred to spend most of their time in the chamber with a mouse (327,3±66,7 s) than in the chamber with a Lego (159,7±48,9 s; p<0,05) or in the central chamber (113,0±25,9 s; p<0,05).Conclusion - dipeptide Pro-Gly is effective when administered in a short course (5-7-fold), with intragastric administration, which distinguishes it from OT (which is administered intranasally). Further studies of Pro-Gly in social disorder are promising.

Pro-Gly, МК-801, zoosocial behavior, mice

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