DNA methylation in human buccal epithelium samples in determining age
Abstract
Based on bioinformatics and statistical analysis of GEO-projects to determine the genome-wide profile of human DNA methylation, a list of CpG dinucleotides with high predictive potential was formed to create models for predicting human age from buccal epithelium samples. The methylation level was determined for 466 samples of individuals from the Republic of Belarus. The correlation coefficients R were calculated, and mathematical models for determining the age of individual were constructed. The average value of the accuracy of age prediction from buccal epithelium samples using 6 CpG-dinucleotides was 4,6 years. The results obtained will be used as a basis for the development of calculators for predicting the age of an individual based on traces of biological character for forensic experts.
About the Authors
V. A. Lemesh
ГНУ «Институт генетики и цитологии» НАН Беларуси
Russian Federation
Russian Federation
V. N. Kipen
ГНУ «Институт генетики и цитологии» НАН Беларуси
Russian Federation
Russian Federation
M. V. Bahdanava
ГНУ «Институт генетики и цитологии» НАН Беларуси
Russian Federation
Russian Federation
A. A. Burakova
ГНУ «Институт генетики и цитологии» НАН Беларуси
Russian Federation
Russian Federation
A. G. Bulgak
Государственное учреждение «РНПЦ «Кардиология»
Russian Federation
Russian Federation
A. V. Bayda
ГУО «Белорусская медицинская академия последипломного образования»
Russian Federation
Russian Federation
O. V. Zotova
Государственное учреждение «РНПЦ «Кардиология»
Russian Federation
Russian Federation
M. A. Kruglikova
Государственное учреждение «РНПЦ «Кардиология»
Russian Federation
Russian Federation
O. I. Dobysh
ГНУ «Институт генетики и цитологии» НАН Беларуси
Russian Federation
Russian Federation
V. I. Sakovich
ГНУ «Институт генетики и цитологии» НАН Беларуси
Russian Federation
Russian Federation
References
1. Assessment of DNA Damage by Comet Assay in Buccal Epithelial /j. Sanchez-Alarcon J. [et al.] // Cells: Problems, Achievement, Perspectives, Environmental Health Risk. - 2016. - P. 77-135. DOI:10.5772/61472
2. Генетика - судебной экспертизе Беларуси / А. Кильчевский [и др.] // Наука и инновации. - 2020. - № 10(212). - С. 22-28.
3. Improved age determination of blood and teeth samples using a selected set of DNA methylation markers / B. Bekaert [et al.] // Epigenetics. - 2015. - Vol. 10(10). - P. 922-930. DOI: 10.1080/15592294.2015.1080413
4. Postmortem age estimation via DNA methylation analysis in buccal swabs from corpses in different stages of decomposition-a «proof of principle» study / B.E. Koop [et al.] // International Journal of Legal Medicine. - 2021. - Vol. 135(1). - P. 167-173. DOI: 10.1007/s00414-020-02360-7
5. Epigenetic age predictions based on buccal swabs are more precise in combination with cell type-specific DNA methylation signatures / M. Eipel [et al.] // Aging (Albany NY). - 2016. - Vol. 8(5). - P. 1034-1048. DOI: 10.18632/aging.100972
6. The PedBE clock accurately estimates DNA methylation age in pediatric buccal cells / L.M. McEwen [et al.] // PNAS. - 2020. - Vol. 22;117(38). - P. 23329-23335. DOI: 10.1073/pnas.1820843116
7. Genome-wide analysis of DNA methylation in buccal cells: a study of monozygotic twins and mQTLs /j. Dongen [et al.] // Epigenetics Chromatin. - 2018. - Vol. 25, 11(1). - P. e1-e14. DOI: 10.1186/s13072-018-0225-x
8. Development of the VISAGE enhanced tool and statistical models for epigenetic age estimation in blood, buccal cells and bones / A. Wozniak [et al.] // Aging (Albany NY). - 2021. - Vol.15;13(5). - P. 6459-6484. DOI: 10.18632/aging.202783
9. Impact of genetic ancestry on chronological age prediction using DNA methylation analysis /j. Fleckhaus [et al.] // Forensic Science International: Genetics. Supplement Series. - 2017. - Vol.6. - P. e399-e400. DOI: 10.1016/j.fsigss.2017.09.162
10. Sperm epigenetics and influence of environmental factors / I. Donkin, R. Barres // Molecular Metabolism. - 2018. - Vol.14. - P. e1-e11. DOI: 10.1016/j.molmet.2018.02.006
11. DNA methylation-based age prediction from saliva: High age predictability by combination of 7 CpG markers / S. R. Hong [et al.] // Forensic Science International: Genetics. - 2017. - Vol.29. - P. 118-125. DOI: 10.1016/j.fsigen.2017.04.006
12. Предсказательный потенциал CpG-маркеров для определения хронологического возраста человека / В.Н. Кипень [и др.] // Молекулярная и прикладная генетика. - 2020. - №.28. - С. 70-80.
Review
For citations:
Lemesh V.A., Kipen V.N., Bahdanava M.V., Burakova A.A., Bulgak A.G., Bayda A.V., Zotova O.V., Kruglikova M.A., Dobysh O.I., Sakovich V.I. DNA methylation in human buccal epithelium samples in determining age. Medical and Biological Problems of Life Activity. 2021;(2):44-52. (In Russ.)
Views: 105
Email this article 