DNA methylation in human buccal epithelium samples in determining age
Abstract
Based on bioinformatics and statistical analysis of GEO-projects to determine the genome-wide profile of human DNA methylation, a list of CpG dinucleotides with high predictive potential was formed to create models for predicting human age from buccal epithelium samples. The methylation level was determined for 466 samples of individuals from the Republic of Belarus. The correlation coefficients R were calculated, and mathematical models for determining the age of individual were constructed. The average value of the accuracy of age prediction from buccal epithelium samples using 6 CpG-dinucleotides was 4,6 years. The results obtained will be used as a basis for the development of calculators for predicting the age of an individual based on traces of biological character for forensic experts.
About the Authors
V. A. Lemesh
ГНУ «Институт генетики и цитологии» НАН Беларуси
Russian Federation
Russian Federation
V. N. Kipen
ГНУ «Институт генетики и цитологии» НАН Беларуси
Russian Federation
Russian Federation
M. V. Bahdanava
ГНУ «Институт генетики и цитологии» НАН Беларуси
Russian Federation
Russian Federation
A. A. Burakova
ГНУ «Институт генетики и цитологии» НАН Беларуси
Russian Federation
Russian Federation
A. G. Bulgak
Государственное учреждение «РНПЦ «Кардиология»
Russian Federation
Russian Federation
A. V. Bayda
ГУО «Белорусская медицинская академия последипломного образования»
Russian Federation
Russian Federation
O. V. Zotova
Государственное учреждение «РНПЦ «Кардиология»
Russian Federation
Russian Federation
M. A. Kruglikova
Государственное учреждение «РНПЦ «Кардиология»
Russian Federation
Russian Federation
O. I. Dobysh
ГНУ «Институт генетики и цитологии» НАН Беларуси
Russian Federation
Russian Federation
V. I. Sakovich
ГНУ «Институт генетики и цитологии» НАН Беларуси
Russian Federation
Russian Federation
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Review
For citations:
Lemesh V.A., Kipen V.N., Bahdanava M.V., Burakova A.A., Bulgak A.G., Bayda A.V., Zotova O.V., Kruglikova M.A., Dobysh O.I., Sakovich V.I. DNA methylation in human buccal epithelium samples in determining age. Medical and Biological Problems of Life Activity. 2021;(2):44-52. (In Russ.)
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