Comparative analysis of the production of soluble factors by mesenchymal stem cells of the placenta-umbilical cord complex, adipose tissue and bone marrow

Mesenchymal stem cells (MSCs) are adult multipotent cells capable of self-renewal and differentiation into various cell types. MSCs can be isolated from various sources, such as the placenta-umbilical cord complex, bone marrow, adipose tissue, etc. The aim of this study was to evaluate the production of cytokines and growth factors (IL-6, HGF, NGF, VEGF-A, MCP-1, sVCAM-1, MCP-3, BDNF, sICAM, TGFβ, P-selectin and E-selectin) in the supernatants of MSC cultures obtained from placenta-umbilical cord complex tissues (decidual tissue, chorionic plate, chorionic villi, amniotic membrane and umbilical cord), adipose tissue and bone marrow. MSCs obtained from different sources showed significant differences in the levels of secretion of produced growth factors and cytokines.

1. Interactions of chemokines and chemokine receptors mediate the migration of mesenchymal stem cells to the impaired site in the brain after hypoglossal nerve injury / J. Ji, B. He, S. Dheen [et al.] // Stem Cells. – 2004. – Vol. 22, № 3. – P. 415-427.

2. The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities / A. Ponte, E. Marais, N. Gallay [et al.] // Stem Cells. – 2007. – Vol. 25, № 7. – P. 1737-1745.

3. Matsumoto, K. Hepatocyte growth factor (HGF) as a tissue organizer for organogenesis and regeneration / K. Matsumoto, T. Nakamura // Biochem Biophys Res Commun. – 1997. – Vol. 239, №3. – P. 639-644.

4. Hepatocyte growth factor inhibits CNS autoimmunity by inducing tolerogenic dendritic cells and CD25 + Foxp3+ regulatory T cells / M. Benkhoucha, M. Santiago-Raber, G. Schneiter [et al.] // Proc Natl Acad Sci U S A. – 2010. – Vol. 107, №14. – P. 6424-6429.

5. Intracellular role of IL-6 in mesenchymal stromal cell immunosuppression and proliferation / А. Dorronsoro, V. Lang, I. Ferrin [et al.] // Sci Rep. – 2020. – Vol. 10, №1. – P. 1-12.

6. Expression of inflammatory cytokines in mesenchymal stem cells derived from proximal humerus fractures / M. M. Viveiros, M. E. Viveiros, M. Silva [et al.] // Stem Cell Investig. – 2022. – Vol. 9, №3. – P. 1-18.

7. Song, N. Mesenchymal stem cell immunomodulation: mechanisms and therapeutic potential / N. Song, M. Scholtemeijer, K. Shah // Trends Pharmacol Sci. – 2020. – Vol. 41, №9. – P. 653-664.

8. Concise review: mesenchymal stem cells: their phenotype, differentiation capacity, immunological features, and potential for homing / G. Chamberlain, J. Fox, B. Ashton [et al.] // Stem Cells. – 2007. – Vol. 25, №11. – P. 2739-2749.

9. Comparative analysis of chemokine receptor›s expression in mesenchymal stem cells derived from human bone marrow and adipose tissue / K. Ahmadian, A. Bahrami, M. Ebrahimi [et al.] // J Mol Neurosci. – 2011. – Vol. 44, №3. – P. 178-185.

10. Eseonu, O. Homing of mesenchymal stem cells: mechanistic or stochastic? Implications for targeted delivery in arthritis / O. Eseonu, C. De Bari // Rheumatology (Oxford). – 2015. – Vol. 54, №2. – P. 210-218.

11. Nerve growth factor (NGF) and NGF receptors in mesenchymal stem/stromal cells: Impact on potential therapies / Z. Kangkang, Y. Yu, T. Guangzhao [et al.] // Stem Cells Transl Med. – 2021. – Vol. 10, №7. – P. 1008-1020.

12. Mesenchymal stem/stromal cells as a pharmacological and therapeutic approach to accelerate angiogenesis / A. Bronckaers, P. Hilkens, W. Martens [et al.] // Pharmacol Ther. – 2014. – Vol. 143, №2. – P. 181-196.

13. Heterogeneity of proangiogenic features in mesenchymal stem cells derived from bone marrow, adipose tissue, umbilical cord, and placenta / W. J. Du, Y. Chi, Z. X. Yang [et al.] // Stem Cell Res Ther. – 2016. – Vol. 7, №1. – Article 163.

14. Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton’s jelly / P. R. Amable, M. V. Teixeira, R. B. Carias [et al.] // Stem Cell Res Ther. – 2014. – Vol. 5, №2. – Article 53.

15. Mesenchymal stem cells and interleukin-6 attenuate liver fibrosis in mice / G. A. Nasir, S. Mohsin, M. Khan [et al.] // J Transl Med. – 2013. – Vol 11. – Article 78.

16.

17.