Activation of type II cannabinoid receptors as variant for mesenchymal stem cell potentiation in a model of peripheral neuropathic pain
https://doi.org/10.58708/2074-2088.2023-2(30)-29-39
Abstract
Transplantation of adipose-derived mesenchymal stem cells (ADMSCs) is currently considered as one of the treatment methods of peripheral neuropathic pain. The analgesic effect of ADMSCs has been proven, and one of the potential options for its enhancement and prolongation could be the activation of type 2 cannabinoid receptors (CB2 receptors). The antinociceptive and reparative effects of ADMSCs were analyzed after type 2 cannabinoid receptors (CB2) pharmacological stimulation by the selective agonist AM1241 on the ADMSC’s membranes and in the tissues of the area of sciatic nerve traumatic injury in rats. It was found that pharmacological stimulation of CB2 receptors on ADMSC’s membranes accelerated the recovery of nociceptive sensitivity to mechanical and thermal stimuli compared with transplantation of ADMSCs alone. Early (before transplantation of ADMSCs) activation of CB2 receptors in tissues surrounding the sciatic nerve more effectively attenuated nociceptive responses to thermal stimuli in the long-term period of the experiment. Stimulation of CB2 receptors did not significantly affect the rate of recovery of gait parameters in rats after ADMSCs transplantation, but led to the structure recovery of the sciatic nerve distal segment of rats. The latter was expressed in increased proliferation of Schwann cells and a decrease in the number of damaged nerve fibers.
About the Authors
A.-M. Yerofeyeva
Институт физиологии НАН Беларуси
Belarus
Belarus
S. Pinchuk
Институт биофизики и клеточной инженерии НАН Беларуси
Belarus
Belarus
S. Ryabceva
Институт физиологии НАН Беларуси
Belarus
Belarus
A. Molchanova
Институт физиологии НАН Беларуси
Belarus
Belarus
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For citations:
Yerofeyeva A., Pinchuk S., Ryabceva S., Molchanova A. Activation of type II cannabinoid receptors as variant for mesenchymal stem cell potentiation in a model of peripheral neuropathic pain. Medical and Biological Problems of Life Activity. 2023;(2):29-39. (In Russ.) https://doi.org/10.58708/2074-2088.2023-2(30)-29-39
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