Nanoarchitectonics and nanomechanical properties of the surface of human and mouse erythrocytes of the BALB/c line after irradiation of whole blood with x-ray radiation at a dose of 0,5 Gy

Despite the fact that erythrocytes are considered relatively resistant to the action of ionizing radiation, the recent literature data have indicated a presence of changes in their properties, detected by modern methods when the cell are irradiated at relatively low doses. The aim of the work was a comparative analysis of changes in the parameters of structural and nanomechanical properties of the surface of human and Balb/c line mouse erythrocytes when whole blood was irradiated in vitro with X-ray radiation at a dose of 0,5 Gy using the atomic force microscopy in PeakForce QNM mode. There were no significant differences in the roughness of the topographic images and the adhesion force maps of the areas of the erythrocyte surface with a size of 250 nm × 250 nm for humans and mice, as well as its changes after the action of X-ray radiation. For nanoscale areas of the cell surface, an increase in the average values of the adhesion force and the spatial period corresponding to the average cell size of the membrane skeleton network of erythrocytes after the X-ray irradiation of blood was found. The obtained data indicate low-dose radiation-induced changes in the parameters of the adhesive properties and nanoarchitectonics of the cell surface that areassociated with changes in the spatial structure of the membrane skeleton of erythrocytes.

Atomic force microscopy, red blood cells, X-ray radiation, human, mouse, nanomechanical properties

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