The using of synthetic oligopeptide for binding and removal of IL-6 from blood plasma
Abstract
The article presents the results of an experimental study of the IL-6 interaction with the synthetic peptide Ser-Phe-Tyr-Arg, which is a structural analogue of the soluble IL-6 receptor (gp80). The interaction of the peptide with the cytokine was been evaluated by the change in the concentration of IL-6, which was assessed by enzyme immunoassay. The binding efficiency was been calculated based on a statistical analysis of the dependence of the concentration of the complex IL-6 with the peptide on the initial concentration of the cytokine. Studies have shown that Ser-Phe-Tyr-Arg, both in free and immobilized form, has the ability to bind IL-6. The results obtained are the basis for the development of medical products for hemosorption in order to extract IL-6 from human blood plasma.
About the Authors
T. V. Ryabtseva
УО «Белорусский государственный медицинский университет»
Russian Federation
Russian Federation
A. D. Taganovich
УО «Белорусский государственный медицинский университет»
Russian Federation
Russian Federation
D. A. Makarevich
УО «Белорусский государственный медицинский университет»
Russian Federation
Russian Federation
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Review
For citations:
Ryabtseva T.V., Taganovich A.D., Makarevich D.A. The using of synthetic oligopeptide for binding and removal of IL-6 from blood plasma. Medical and Biological Problems of Life Activity. 2022;(2):99-104. (In Russ.)
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