Assessment of oxidative stress in determining the probability of developing early renal allograft dysfunction

Abstract

We have determined the risk of developing renal alograft dysfunction in patients with chronic kidney disease at organ transplantation based on an assessment of the total plasma antioxidant capacity. The pro/antioxidant balance of the blood plasma of recipients was studied before surgery and 24 hours after by the method of luminol-dependent chemiluminescence (LDCL) on a fluoriometer/spectrophotometer. We evaluated the main indicator of LDCL luminescence intensity (Imax). According to the study results, Imax before surgery in patients with RAD did not differ from recipients with NGF. As early as 24 hours after kidney transplantation, there was a significant decrease in the level of pro/antioxidant balance in blood plasma in both groups of patients. On the seventh day, it was revealed that 17 patients had allograft dysfunction, and 30 had normal function. At the same time, after 24 hours the Imax level was higher in the group of patients with NGF than in the RAD group. Thus, assessment of the total antioxidant capacity of the plasma in patients in the early post-transplant period makes it possible to determine the probability of developing renal allograft dysfunction.