Preclinical stage of testing new wound dressings in an animal experiment: dynamics of wound healing and the levels of growth factors
https://doi.org/10.58708/2074-2088.2025-1(33)-79-88
Abstract
Treatment of patients with trophic disorders due to diabetes mellitus is an important medical and social problem, since decompensation and progression of the disease and its complications often lead to disability of patients. The presence of long-term non-healing trophic ulcers requires daily, often painful, dressings, which significantly reduces the quality of life of patients. Therefore, the development of composite hydrogel coatings of prolonged action will solve one of the most important problems of modern surgery. The article presents the results of a biochemical analysis of the blood of laboratory rats with the determination of the level of fibroblast growth factor-2 and endothelin-1 in dynamics against the background of the use of the developed hydrogel wound coatings of prolonged action. Based on the obtained data, the optimal composition of wound dressing for the treatment of trophic ulcers against the background of ischemia in diabetes mellitus was determined.
Keywords
wound dressings,
wound healing,
endothelin-1,
fibroblast growth factor,
diabetes mellitus,
trophic ulcer
УДК: 616-001.4-089-092.9:57.084
About the Authors
V. I. Silvistrovich
Gomel State Medical University Educational Institution
Belarus
Belarus
Gomel
A. A. Lyzikov
Bradford teaching hospital NHS Foundation Trus
United Kingdom
United Kingdom
Bradford
Yu. I. Yarets
State Institution "Russian Scientific Research Center for Radiation Medicine and Human Ecology"
Belarus
Belarus
Gomel
References
1. Supil'nikov, A.A. Morfologicheskie i fiziologicheskie aspekty techeniya ranevogo processa / A.A. Supil'nikov, A.A. Devyatkin, O.N. Pavlova, O.N. Gulenko // Vestnik medicinskogo instituta «REAVIZ». – 2016. – T.3. – S. 144-151.
2. Sidorenko, N.K. Rol' osnovnogo faktora rosta fibroblastov v razvitii i progressirovanii diabeticheskih mikroangiopatij u bol'nyh saharnym diabetom 1 tipa / N.K. Sidorenko // Vestnik ural'skoj medicinskoj akademicheskoj nauki. – 2008. – №4. – S. 108–110.
3. Florova, M.S. Rol' rostovyh faktorov v patogeneze endometrioza / M.S. Florova // ZHurnal akusherstva i zhenskih boleznej. — 2019. — T. 68. — No 3. — S. 71–80. https://doi.org/10.17816/JOWD68371-80
4. Presta M. Inflammatory cells and chemokines sustain FGF2-induced angiogenesis / M. Presta, G. Andrés, D. Leali [et al] // Eur Cytokine Netw. – 2009. – V.20, №2. – Rr. 39–50.
5. Dvorak P. Basic fibroblast growth factor and its receptors in human embryonic stem cells / R. Dvorak, A.Hampl // Folia Histochem Cytobiol. – 2005. – V.43, №4. – Rr. 203–208
6. Vincent TL. Fibroblast growth factor 2: good or bad guy in the joint? // Arthritis Res Ther. – 2011. – V.13, №5. – R.127.
7. Basic fibroblast growth factor upregulates the expression of vascular endothelial growth factor in vascular smooth muscle cells synergistic interaction with hypoxia / G.T. Stavri [et al.] // Circulation. — 1995. — Vol. 92, No 1. — P. 11 — 14.
8. Fibroblast Growth Factor Receptors (FGFRs): Structures and Small Molecule Inhibitors / S. Dai, Z. Zhou, Z. Chen, G. Xu, Y. Chen // Cells. 2019. Vol. 8, No 6. P. 614. DOI: 10.3390/cells8060614.
9. Yun Y.R. Fibroblast growth factors: biology, function, and application for tissue regeneration / Y.R. Yun, J. E. Won [et al] // J Tissue Eng. – 2010. doi: 10.4061/2010/218142.
10. Nikitenko, V.I. Ispol'zovanie faktorov rosta fibroblastov dlya lecheniya ran i ozhogov / V.I. Nikitenko, S.A. Pavlovichev, V.S. Polyakova i dr. // Hirurgiya. ZHurnal im. N.I. Pirogova. – 2012. – T.12. – S. 72–76.
11. Zorin, V.L. Dermal'nye fibroblasty dlya lecheniya defektov kozhi / V.L. Zorin, A.I. Zorina, O.S. Petrakova, V.R. CHerkasov // Kletochnaya transplantologiya i tkanevaya inzheneriya. – 2009. – T.4. – №4. – S. 26-40.
12. Yoshida A. Differential endothelial migration and proliferation to basic fibroblast growth factor and vascular endothelial growth factor / A. Yoshida, B. Anand-Apte, B.R. Zetter // Growth Factors. — 1996. — Vol. 13, No1 — 2. — P. 57 — 64.
13. Srubilin, D.V. Sostoyanie endoteliya sosudov u krys pri hronicheskoj intoksikacii dihloretanom / D.V. Srubilin, D.A. Enikeev, V.A. Myshkin // Sovremennye problemy nauki i obrazovaniya. – 2016. – № 5. URL: https://science-education.ru/ru/article/view?id=25382 (data obrashcheniya: 18.09.2022).
14. Endotelin-sovremennyj biomarker serdechno-sosudistyh zabolevanij / E. Z. Goluhova, A. M. Alieva, T. T. Kakuchaya, G. G. Arakelyan, N. I. Bulaeva // Kreativnaya kardiologiya. – 2011. – T.5, №2. ¬ S. 97–103/
15. Ivanov, A. N. Vliyanie polilaktidnyh ranevyh pokrytij s taninovoj kislotoj na koncentraciyu markerov angiogeneza, al'teracii i aktivacii endotelial'nyh kletok v krovi u belyh krys pri zazhivlenii eksperimental'nogo polnoslojnogo defekta kozhi / A.N. Ivanov, M.A. Sahan', A.V. Ermakov [i dr.] // Regionarnoe krovoobrashchenie i mikrocirkulyaciya. – 2024. – T.23, №3. – S.81–88. Doi: 10.24884/1682-6655-2024-23-3-81-88.
16. Mitryashov, K.V. Sravnitel'noe issledovanie effektivnosti ranevyh pokrytij na osnove gialuronovoj kisloty i atravmaticheskih povyazok v mestnom lechenii pogranichnyh ozhogov / K.V. Mitryashov, V.V. Usov, V.A. SHarkova // ZHurnal im. N.V. Sklifosovskogo «Neotlozhnaya medicinskaya pomoshch'». – 2021. – T.10, №4. – S.695-701. https://doi.org/10.23934/2223-9022-2021-10-4-695-701.
17. Wang, F. Effect of natural-based biological hydrogels combined with growth factors on skin wound healing / Feng Wang, Yu Gao, Hao Li [et all] // Nanotechnology Reviews. – 2022. – vol. 11, №.1. – pp. 2493-2512. https://doi.org/10.1515/ntrev-2022-0122
18. Maksimova, N.V. Patofiziologicheskie aspekty processa zazhivleniya ran v norme i pri sindrome diabeticheskoj stopy / N.V. Maksimova, A.V. Lyundup, R.O. Lyubimov [i dr.] // Vestnik RAMN. – 2014. – T.11-12. – S.110–117.
19. Komelyagina, E.YU. Faktory rosta v terapii yazvennyh defektov pri sindrome diabeticheskoj stopy / E.YU. Komelyagina, M.B Anciferov // Saharnyj diabet. — 2019. — T. 22, No4. — S. 377-383. doi: 10.14341/DM10130
20. Baranov, E.V. Ispol'zovanie mezenhimal'nyh stvolovyh kletok zhirovoj tkani pri lechenii ranevyh defektov kozhnyh pokrovov v eksperimente / E.V. Baranov, S.I. Tret'yak, M.K. Nedz'ved', I.B. Vasilevich, E.S. Lobanok, I.D. Volotovskij // Vescі Nacyyanal'naj akademіі navuk Belarusі. – 2014. - №1. - S.60-67
21. Blednov, A.V. Razrabotka i primenenie immobilizovannyh form proteoliticheskih fermentov v medicine / A.V. Blednov // Vestnik farmacii. – 2005. -T.27, №1. - S. 9-18
22. Corsetti, G. Topical application of dressing with amino acids improves cutaneous wound healing in aged rats / Giovanni Corsettia, Giuseppe D’Antonab, Francesco Saverio Dioguardic, Rita Rezzani // Acta Histochem. – 2010 – V.112, №5. – Rr.97-507 doi:10.1016/j.acthis.2009.05.003
23. Melamed, V. D. Effektivnost' ranevyh pokrytij s nanovoloknami hitozana v sochetanii s nekrektomiej pri lechenii otmorozhenij v eksperimente / V. D. Melamed, A. L. Valentyukevich, N. I. Prokopchik // Ves. Nac. akad. navuk Belarusі. Ser. med. navuk. – 2022. – T. 19, No 2. – S. 248–256. https://doi.org/10.29235/1814-6023- 2022-19-2-248-256
24. Zinov'ev, E.V. Ocenka effektivnosti ranevyh pokrytij na osnove bakterial'noj cellyulozy s fukoidanom pri ozhogah kozhi / E.V. Zinov'ev, S.A. Luk'yanov, V.N. Cygan [i dr] // Vestnik Rossijskoj voenno-medicinskoj akademii – 2019 – T.65, №1. – S.148–152
25. Novosad, Y.A. The Wound-Healing Effect of a Novel Fibroblasts-Impregnated Hydroxyethylcellulose Gel in a Rat Full-Thickness Burn Model: A Preclinical Study. / Y.A. Novosad, A.S. Shabunin, N.I. Enukashvily [et al] // Biomedicines. – 2024. – V.12, №10. – 2215. https://doi.org/10.3390/ biomedicines12102215
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For citations:
Silvistrovich V.I., Lyzikov A.A., Yarets Yu.I. Preclinical stage of testing new wound dressings in an animal experiment: dynamics of wound healing and the levels of growth factors. Medical and Biological Problems of Life Activity. 2025;(1):79-88. (In Russ.) https://doi.org/10.58708/2074-2088.2025-1(33)-79-88
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