medbioМедико-биологические проблемы жизнедеятельностиMedical and Biological Problems of Life Activity2074-2088Республиканский научно-практический центр радиационной медицины и экологии человека10.58708/2074-2088.2025-3(35)-34-41medbio-469Research ArticleМЕДИКО-БИОЛОГИЧЕСКИЕ ПРОБЛЕМЫMEDICAL-BIOLOGICAL PROBLEMSСравнительный анализ продукции растворимых факторов мезенхимальными стволовыми клетками плацентарно-пуповинного комплекса, жировой ткани и костного мозгаComparative analysis of the production of soluble factors by mesenchymal stem cells of the placenta-umbilical cord complex, adipose tissue and bone marrowЮркинаЕ. Г.YurkinaE. G.

г. Минск

КривенкоС. И.KrivenkoS. I.

г. Минск

ПримаковаЕ. А.PrimakovaE. A.

г. Минск

НазароваЕ. А.NazarovaE. A.

г. Минск

СымановичА. А.SymanovichA. A.

г. Минск

ДедюляН. И.DedyulyaN. I.

г. Минск

РомановаИ. А.RomanovaI. A.

г. Минск

ЯнушевскаяЕ. А.YanushevskayaE. A.

г. Минск

СмольниковаВ. В.SmolnikovaV. V.

г. Минск

ЕфимовД. Ю.EfimovD. Yu.

г. Минск

ГУ «Минский НПЦ хирургии, трансплантологии и гематологии»ГУ «Минский НПЦ хирургии, трансплантологии и гематологии»202520102025033441Copyright © Юркина Е.Г., Кривенко С.И., Примакова Е.А., Назарова Е.А., Сыманович А.А., Дедюля Н.И., Романова И.А., Янушевская Е.А., Смольникова В.В., Ефимов Д.Ю., 20252025Юркина Е.Г., Кривенко С.И., Примакова Е.А., Назарова Е.А., Сыманович А.А., Дедюля Н.И., Романова И.А., Янушевская Е.А., Смольникова В.В., Ефимов Д.Ю.Yurkina E.G., Krivenko S.I., Primakova E.A., Nazarova E.A., Symanovich A.A., Dedyulya N.I., Romanova I.A., Yanushevskaya E.A., Smolnikova V.V., Efimov D.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://medbio.ejournal.by/jour/article/view/469

Целью данного исследования являлась оценка продукции цитокинов и факторов роста (IL-6 (interleukin-6, интерлейкин-6), HGF (hepatocyte growth factor, фактор роста гепатоцитов), NGF (nerve growth factor, фактор роста нервов), VEGF-А (vascular endothelial growth factor A, фактор роста эндотелия сосудов А), MCP-1 (monocyte chemotactic protein-1, моноцитарный хемоаттрактантный протеин-1), sVCAM-1 (soluble vascular cell adhesion molecule-1, растворимая молекула адгезии сосудистых клеток-1), MCP-3 (monocyte chemotactic protein 3, моноцитарный хемоаттрактантный протеин-3), sICAM-1 (soluble intercellular adhesion molecule-1, растворимая молекула межклеточной адгезии-1, TGFβ (transforming growth factor beta, трансформирующий фактор роста бета), P-selectin (P-selectin, Р-селектин) и E-selectin (Е-selectin, Е-селектин)) в супернатантах культур мезенхимальных стволовых клеток (МСК), полученных из тканей плацентарно-пуповинного комплекса (децидуальной ткани, хориальной пластинки, ворсинок хориона, амниотической мембраны и пупочного канатика), жировой ткани и костного мозга. Установлено, что МСК, полученные из различных тканевых источников, значительно отличаются по уровням секреции продуцируемых ростовых факторов и цитокинов. Полученные результаты обосновывают выбор оптимального тканевого источника для получения биомедицинских клеточных продуктов на основе МСК человека с улучшенными целевыми свойствами для повышения регенераторного и противовоспалительного эффекта клеточной терапии.

Mesenchymal stem cells (MSCs) are adult multipotent cells capable of self-renewal and differentiation into various cell types. MSCs can be isolated from various sources, such as the placenta-umbilical cord complex, bone marrow, adipose tissue, etc. The aim of this study was to evaluate the production of cytokines and growth factors (IL-6, HGF, NGF, VEGF-A, MCP-1, sVCAM-1, MCP-3, BDNF, sICAM, TGFβ, P-selectin and E-selectin) in the supernatants of MSC cultures obtained from placenta-umbilical cord complex tissues (decidual tissue, chorionic plate, chorionic villi, amniotic membrane and umbilical cord), adipose tissue and bone marrow. MSCs obtained from different sources showed significant differences in the levels of secretion of produced growth factors and cytokines.

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The authors declare that there are no conflicts of interest present.