medbioМедико-биологические проблемы жизнедеятельностиMedical and Biological Problems of Life Activity2074-2088Республиканский научно-практический центр радиационной медицины и экологии человека10.58708/2074-2088.2023-2(30)-69-73medbio-314Research ArticleМЕДИКО-БИОЛОГИЧЕСКИЕ ПРОБЛЕМЫMEDICAL-BIOLOGICAL PROBLEMSМетод мультиплексной полимеразной цепной реакции для диагностики вирусной кишечной инфекции неуточненнойMultiplex polymerase chain reaction method for the diagnosis of unspecified viral acute gastroenteritishttps://orcid.org/0000-0001-6431-5050ПоклонскаяН. В.PaklonskayaN. V.

Ведущий научный сотрудник

г. Минск

Minsk

labsanvir@gmail.comhttps://orcid.org/0000-0002-4521-6599ШиловаЮ. А.ShilovaYu. A.

младший научный сотрудник

г. Минск

Leading Researcher

Minsk

 

Jusa-89@yandex.ruhttps://orcid.org/0000-0001-7309-152XАмвросьеваТ. В.AmvrosievaT. V.

д.м.н., профессор, зав. лабораторией инфекций с природным резервуаром

г. Минск

D. Sc. (Med.), Professor, Head of the Laboratory

Minsk

amvrosieva@gmail.comГУ «РНПЦ эпидемиологии и микробиологии»The Republican Research and Practical Center of Epidemiology and Microbiology202328082023026973Copyright © Поклонская Н.В., Шилова Ю.А., Амвросьева Т.В., 20232023Поклонская Н.В., Шилова Ю.А., Амвросьева Т.В.Paklonskaya N.V., Shilova Y.A., Amvrosieva T.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://medbio.ejournal.by/jour/article/view/314

Острая кишечная инфекция (ОКИ) может быть вызвана широким кругом вирусных патогенов. Детекция доминирующих возбудителей (норо-, рота-, адено-, энтеровирусов) позволяет установить этиологию 40-60% случаев ОКИ. Для увеличения доли их этиологической расшифровки целесообразно проводить исследования в отношении минорных кишечных вирусов. В настоящей статье представлен разработанный нами метод мультиплексной полимеразной цепной реакции для диагностики вирусной кишечной инфекции неуточненной. Он позволяет детектировать генетический материал астро-, сапо-, парэхо-, бока-, пикобирна- и аичи вирусов в биологическом материале (фекалии). Апробация метода позволила детектировать присутствие данных возбудителей у 4,89% пациентов с ОКИ, в биологическом материале которых отсутствовали доминирующие кишечные вирусы.

Acute gastroenteritis (AG) can be caused by a wide range of viral pathogens. Detection of dominant pathogens (noro-, rota-, adeno-, enteroviruses) makes it possible to establish the etiology of 40-60% of AG cases. To increase the proportion of their etiological interpretation, it is advisable to conduct research on minor intestinal viruses. This article presents the method of multiplex polymerase chain reaction for the diagnosis of unspecified viral intestinal infection. It allows detecting the genetic material of astro-, sapo-, parecho-, boka-, picobirna- and aichi viruses in biological material (faeces). The approbation of the method made it possible to detect the presence of these pathogens in 4,89% of patients with AG, in whose biological material were no dominant intestinal viruses.

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The authors declare that there are no conflicts of interest present.