medbioМедико-биологические проблемы жизнедеятельностиMedical and Biological Problems of Life Activity2074-2088Республиканский научно-практический центр радиационной медицины и экологии человекаmedbio-262Research ArticleКЛИНИЧЕСКАЯ МЕДИЦИНАCLINICAL MEDICINEОценка содержания кольцевых молекул ДНК Т- и В-клеточного рецептора (TREC/KREC) у новорожденных различного гестационного возрастаEvaluation of the content of circular DNA molecules T- and B-receptor (TREC / KREC) in newborns of different gestational agesПоляковаЕ. А.PolyakovaE. A.noemail@neicon.ruОстроушкоД. В.OstrouskoD. V.noemail@neicon.ruСтёганцеваМ. В.StegantsevaM. V.noemail@neicon.ruГурьяноваИ. Е.GuryanovaI. E.noemail@neicon.ruТимоховаЮ. В.TsimokhavaY. V.noemail@neicon.ruБелевцевМ. В.BelevtsevM. V.noemail@neicon.ruГУ «РНПЦ детской онкологии, гематологии и иммунологии»Russian FederationГУ «РНПЦ «Мать и дитя»Russian Federation20210203202301135142Copyright © Полякова Е.А., Остроушко Д.В., Стёганцева М.В., Гурьянова И.Е., Тимохова Ю.В., Белевцев М.В., 20232023Полякова Е.А., Остроушко Д.В., Стёганцева М.В., Гурьянова И.Е., Тимохова Ю.В., Белевцев М.В.Polyakova E.A., Ostrousko D.V., Stegantseva M.V., Guryanova I.E., Tsimokhava Y.V., Belevtsev M.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://medbio.ejournal.by/jour/article/view/262

В данном исследовании были определены границы нормальных значений TREC и KREC в периферической крови 58 здоровых доношенных новорожденных. Количество копий TREC составило 3383 - 238848 копии (Q1-Q3), KREC: 2729 - 236953 копии на 1 млн лейкоцитов (Q1-Q3). Исследовано влияние гестационного возраста 117 новорожденных, родившихся преждевременно (22,5-37,8 недель), на количество данных маркеров. Показано, что количество как TREC, так и KREC возрастало с увеличением гестационного возраста. Выявлено, что вес как доношенных, так и недоношенных новорожденных различной гестационной зрелости не влияет на количество TREC и KREC.

In this study, the normal range for TREC and KREC in the peripheral blood of 58 healthy full-term infants was determined. The number of TREC copies was 3383-238848 copies (Q1- Q3), KREC 2729-236953 copies per 1 million leukocytes (Q1-Q3). The influence of the gestational age of 117 newborns born prematurely (22,5-37,8 weeks) on the number of these markers was studied. It was shown that the amount of both TREC and KREC increased with increasing gestational age. It was revealed that the weight of both full-term and premature newborns of different gestational maturity does not affect the number of TREC and KREC

новорожденныеТ- и В-клеточный рецепторTRECKRECПЦР в реальном времениnewbornТ- and В-cell receptorTRECKRECreal-time PCRReferencesPercentiles of Lymphocyte Subsets in Preterm Infants According to Gestational Age Compared to Children and Adolescents / S. Huenecke [et al.] // Human Immunology. - 2016. - № 84. - P. 291-297.Percentiles of Lymphocyte Subsets in Preterm Infants According to Gestational Age Compared to Children and Adolescents / S. Huenecke [et al.] // Human Immunology. - 2016. - № 84. - P. 291-297.Melville, J.M. The immune consequences of preterm birth [Electronic resource] /j.M. Melville, T.J.M. Moss // Frontiers in neurosciense. - 2013. - № 49. - Mode of access: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3659282/pdf/fnins-07-00079.pdf. - Date of access: 31.07.2020.Melville, J.M. The immune consequences of preterm birth [Electronic resource] /j.M. Melville, T.J.M. Moss // Frontiers in neurosciense. - 2013. - № 49. - Mode of access: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3659282/pdf/fnins-07-00079.pdf. - Date of access: 31.07.2020.Durandy A. Ontogeny of the immune system. / A. Dyrandy // Transfusion Medicine and Hemotherapy. - 2003. - № 30. - P. 222- 227.Durandy A. Ontogeny of the immune system. / A. Dyrandy // Transfusion Medicine and Hemotherapy. - 2003. - № 30. - P. 222- 227.Innate immunity in human newborn infants: prematurity means more than immaturity / T. Strunk [et.al.] // The Journal of Maternal-Fetal and Neonatal Medicine. - 2011. - № 24. - P. 25-31.Innate immunity in human newborn infants: prematurity means more than immaturity / T. Strunk [et.al.] // The Journal of Maternal-Fetal and Neonatal Medicine. - 2011. - № 24. - P. 25-31.Особенности врожденного и адаптивного иммунитета недоношенных детей с тяжелым гипоксически-ишемическим поражением центральной нервной системы / Л.С. Устьянцева [и др.] // Российский вестник перинатологии и педиатрии. - 2017. - T. 62, № 3. - С. 59-65.Особенности врожденного и адаптивного иммунитета недоношенных детей с тяжелым гипоксически-ишемическим поражением центральной нервной системы / Л.С. Устьянцева [и др.] // Российский вестник перинатологии и педиатрии. - 2017. - T. 62, № 3. - С. 59-65.Снимщикова, И.А. Курс лекций по прикладной иммунологии: учеб. Пособие / И.А. Снимщикова. - Орел: Орловский гос.мед. ун-т, 2015. - 120 с.Снимщикова, И.А. Курс лекций по прикладной иммунологии: учеб. Пособие / И.А. Снимщикова. - Орел: Орловский гос.мед. ун-т, 2015. - 120 с.Puck, J.M. Laboratory technology for population-based screening for severe combined immunodeficiency in neonates: The winner is T-cell receptor excision circles /j.M. Puck // J Allergy Clin Immunol. - 2012. - Vol. 129. - P. 607-616.Puck, J.M. Laboratory technology for population-based screening for severe combined immunodeficiency in neonates: The winner is T-cell receptor excision circles /j.M. Puck // J Allergy Clin Immunol. - 2012. - Vol. 129. - P. 607-616.Verbsky, J. The Wisconsin approach to newborn screening for severe combined immunodeficiency/j. Verbsky, M. Thakar, J. Routes // Jourlnal of allergy and clinical immunology. - 2012. - Vol. 129. - P. 622-627.Verbsky, J. The Wisconsin approach to newborn screening for severe combined immunodeficiency/j. Verbsky, M. Thakar, J. Routes // Jourlnal of allergy and clinical immunology. - 2012. - Vol. 129. - P. 622-627.Serana, F. Use of V(D)J recombination excision circles to identify T- and B-cell defects and to monitor the treatment in primary and acquired immunodeficiencies / F. Serana, M. Chiarini, C. Zanotti // Journal of translational medicine. - 2013. - Vol. 11. - Р. 1-11.Serana, F. Use of V(D)J recombination excision circles to identify T- and B-cell defects and to monitor the treatment in primary and acquired immunodeficiencies / F. Serana, M. Chiarini, C. Zanotti // Journal of translational medicine. - 2013. - Vol. 11. - Р. 1-11.Newborn screening for severe combined immunodeficiency in 11 screening programs in the United States / A. Kwan [et al.] // JAMA. - 2014. Vol. 312. - P. 729-738.Newborn screening for severe combined immunodeficiency in 11 screening programs in the United States / A. Kwan [et al.] // JAMA. - 2014. Vol. 312. - P. 729-738.Incidence of severe combined immunodeficiency through newborn screening in a Chinese population / YH. Chien [et al.] // Journal Formos Medical Association. - 2015. - Vol. 114. - P. 12-16.Incidence of severe combined immunodeficiency through newborn screening in a Chinese population / YH. Chien [et al.] // Journal Formos Medical Association. - 2015. - Vol. 114. - P. 12-16.First Year of Israeli Newborn Screening for Severe Combined Immunodeficiency - Clinical Achievements and Insights [Electronic resource] / E. Rechavi [et al.] // Frontiers Immunology. - 2017. - Vol. 8. - Mode of access: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5682633/ - Date of access: 31.07.2020.First Year of Israeli Newborn Screening for Severe Combined Immunodeficiency - Clinical Achievements and Insights [Electronic resource] / E. Rechavi [et al.] // Frontiers Immunology. - 2017. - Vol. 8. - Mode of access: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5682633/ - Date of access: 31.07.2020.Newborn screening for severe combined immunodeficiency in Taiwan / Y.- H. Chien [et al.] // International Journal of Neonatal Screening. - 2017. - Vol. 3. - P. 1-12.Newborn screening for severe combined immunodeficiency in Taiwan / Y.- H. Chien [et al.] // International Journal of Neonatal Screening. - 2017. - Vol. 3. - P. 1-12.Nourizadeh, M. Newborn screening using TREC/KREC assay for severe T and B cell lymphopenia in Iran / M. Nourizadeh, L. Shakerian, S. Borte // Scandinavian Journal of Immunology. - 2018. Vol. 88. - P. 1-9.Nourizadeh, M. Newborn screening using TREC/KREC assay for severe T and B cell lymphopenia in Iran / M. Nourizadeh, L. Shakerian, S. Borte // Scandinavian Journal of Immunology. - 2018. Vol. 88. - P. 1-9.First Universal Newborn Screening Program for Severe Combined Immunodeficiency in Europe. Two-Years’ Experience in Catalonia (Spain) [Electronic resource] / A.A. Ramírez [et al.] // Frontiers in Immunology. - 2019. - Mode of access: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6818460/pdf/fimmu-10-02406.pdf. - Date of access: 31.07.2020.First Universal Newborn Screening Program for Severe Combined Immunodeficiency in Europe. Two-Years’ Experience in Catalonia (Spain) [Electronic resource] / A.A. Ramírez [et al.] // Frontiers in Immunology. - 2019. - Mode of access: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6818460/pdf/fimmu-10-02406.pdf. - Date of access: 31.07.2020.Universal newborn screening for severe combained immunodeficiency [Electronic resource] / M. van der Burg [et al.] // Frontiers in Pediatrics. - 2019. - Mode of access: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6759820/pdf/fped-07-00373.pdf. - Date of access: 31.07.2020.Universal newborn screening for severe combained immunodeficiency [Electronic resource] / M. van der Burg [et al.] // Frontiers in Pediatrics. - 2019. - Mode of access: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6759820/pdf/fped-07-00373.pdf. - Date of access: 31.07.2020.The TREC/KREC Assay for the Diagnosis and Monitoring of Patients with DiGeorge Syndrome [Electronic resource] / E. Froňková [et. al.] // PLOS ONE. - 2014. - Vol. 8. - №12. - Mode of access: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259354/pdf/pone.0114514.pdf. - Date of access: 1.08.2020.The TREC/KREC Assay for the Diagnosis and Monitoring of Patients with DiGeorge Syndrome [Electronic resource] / E. Froňková [et. al.] // PLOS ONE. - 2014. - Vol. 8. - №12. - Mode of access: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259354/pdf/pone.0114514.pdf. - Date of access: 1.08.2020.Thymic output: a bad TREC record / M.D. Hazenberg [et al.] // Nature immunology. - 2003. - Vol.4. - P. 97-99.Thymic output: a bad TREC record / M.D. Hazenberg [et al.] // Nature immunology. - 2003. - Vol.4. - P. 97-99.Determining Laboratory Reference Values of TREC and KREC in Different Age Groups of Iranian Healthy Individuals / L. Shakerian [et.al.] // Iranian Journal of Allergy Asthma and Immunology. - 2019. - Vol. 2. - № 18. - P. 143-152.Determining Laboratory Reference Values of TREC and KREC in Different Age Groups of Iranian Healthy Individuals / L. Shakerian [et.al.] // Iranian Journal of Allergy Asthma and Immunology. - 2019. - Vol. 2. - № 18. - P. 143-152.Определение референсных интервалов TREC и KREC для скрининга новорожденных с иммунодефицитными состояниями в РФ / М.А. Гордукова [и др.] // Медицинская иммунология. - 2019. - Т. 21, № 3. - С. 527-538.Определение референсных интервалов TREC и KREC для скрининга новорожденных с иммунодефицитными состояниями в РФ / М.А. Гордукова [и др.] // Медицинская иммунология. - 2019. - Т. 21, № 3. - С. 527-538.Neonatal screening for severe combined immunodeficiency in Brazil / M Kanegae [et al.] // Jornal de Pediatria. - 2016. - № 92. - P. 374-380.Neonatal screening for severe combined immunodeficiency in Brazil / M Kanegae [et al.] // Jornal de Pediatria. - 2016. - № 92. - P. 374-380.Newborn Screening for SCID in New York State: Experience from the First Two Years [Electronic resource] / B.H. Vogel [et al.] // Journal of Clinical Immunology. - 2014. - № 3. - Mode of access: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4090801/pdf/nihms595483.pdf. - Date of access: 1.08.2020.Newborn Screening for SCID in New York State: Experience from the First Two Years [Electronic resource] / B.H. Vogel [et al.] // Journal of Clinical Immunology. - 2014. - № 3. - Mode of access: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4090801/pdf/nihms595483.pdf. - Date of access: 1.08.2020.Дерябина, С.С Определение нормативных значений TREC и KREC в сухих пятнах крови новорожденных разного срока гестации в Свердловской области / С.С. Дерябина, И.А. Тузанкина, В.Н. Шершнев // Медицинская иммунология. - 2018. - Т. 20, № 1. - C. 85-98.Дерябина, С.С Определение нормативных значений TREC и KREC в сухих пятнах крови новорожденных разного срока гестации в Свердловской области / С.С. Дерябина, И.А. Тузанкина, В.Н. Шершнев // Медицинская иммунология. - 2018. - Т. 20, № 1. - C. 85-98.Timely and spatially regulated maturation of B and T cell repertoire during human fetal development [Electronic resource] / E. Rechavi [et al.] // Science Translational Medicine. - 2015. № 276. - Mode of access: https://pubmed.ncbi.nlm.nih.gov/25717098/ - Date of access: 04.08.2020.Timely and spatially regulated maturation of B and T cell repertoire during human fetal development [Electronic resource] / E. Rechavi [et al.] // Science Translational Medicine. - 2015. № 276. - Mode of access: https://pubmed.ncbi.nlm.nih.gov/25717098/ - Date of access: 04.08.2020.Zelm, M.C. Replication history of B lymphocytes reveals homeostatic proliferation and extensive antigen-induced B-cell expansion / M.C. Zelm, M. van der Burg, J.J. M. van Dongen //The Journal of experimental medicine. - 2007. - Vol. 204. - P. 645- 655.Zelm, M.C. Replication history of B lymphocytes reveals homeostatic proliferation and extensive antigen-induced B-cell expansion / M.C. Zelm, M. van der Burg, J.J. M. van Dongen //The Journal of experimental medicine. - 2007. - Vol. 204. - P. 645- 655.

The authors declare that there are no conflicts of interest present.